FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_86.35200000039bbf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_86.35200000039bbf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127191
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23140.20528907700647006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAC455.4667424E-419.99985313
GCCAGTC653.4378955E-416.1530117
CTAGCCT859.2074715E-615.8815314
GTGTACG600.003756262614.9985589
ACCCGCT750.001018322513.9992768
CTTAGTC902.653036E-413.3326433
CGTGAGC800.001656464713.12490412
TCGTGCT1151.0939846E-513.0422249
ACCTAAT3200.012.6578431
TAGGTAG1804.6729838E-912.4993525
GGTAGTC1857.021299E-912.1615327
CGGGAGC1006.449198E-411.99991126
TGTTAGG1006.451603E-411.9993782
TGGACTG1303.845366E-511.5378655
CTAATAC3000.011.4994053
AGACTGC1059.7147416E-411.427986
TTAGGTA2659.094947E-1211.32016854
ACTGCTC1202.3451583E-411.2494178
TAGTCAG1755.478123E-711.1417879
GGCTATA1100.001431142710.9104621
Produced by FastQC (version 0.11.2)