FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_85.35200000039b7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_85.35200000039b7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465104
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14080.3027279920189893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGGT300.001226250724.9972234
CCGTGAA400.00650897718.7479179
AGACTAT500.001109717.9986
TCTACTC653.4340218E-416.1520523
GCCCCAA600.003752219514.9983341
GTATCCT600.003752219514.9983344
GCCGTTC650.006383211413.8446168
GTATATG1106.8961544E-613.6348491
ACCTAAT1004.2642634E-513.49851
AACCCGT800.001655211613.1235426
TAGTCCG800.001655211613.1235429
CCTAATA1056.739989E-512.8557152
TCAGATG850.002610403612.35156912
ACTATGC900.00400427811.6653718
GTATTAG900.00400427811.6653711
TATACTG1551.5385431E-611.6116135
ATAGTCC1059.700822E-411.4273018
ATACTGT1602.254248E-611.248756
TTATACA1501.3560835E-510.9987784
TATTGGG1100.001431230310.9078792
Produced by FastQC (version 0.11.2)