FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_84.35200000039b39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_84.35200000039b39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757427
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14690.19394608325290755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCCC400.0065049418.75177415
TCCGCTG600.003751632515.00042927
CTGAGCG600.003754929614.9984489
GGTAGTC901.581683E-514.9984487
TAGTCAG800.001656789913.1236439
GTATTAG1302.7560163E-612.6918331
GTCATAC954.185812E-412.6311061
TGGACTA900.004008022611.665465
TATTAGG1806.255323E-811.665462
AGCAGTC900.004008022611.665467
ACGTAGG950.005995077511.05221751
TCCTAAC950.005998024311.0514882
TATGCCT1100.001432940610.9079634
GTATAAA3101.8189894E-1210.6447641
TATACTG3101.8189894E-1210.6440615
GTCTTAC1000.00877487610.4996071
GTTAGAT1000.00877917210.4989143
CTGGACT1000.00877917210.4989144
CCTTATA1451.16642994E-410.3437582
TAGGTAG1356.955919E-49.9989665
Produced by FastQC (version 0.11.2)