FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_83.35200000039af4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_83.35200000039af4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881984
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20800.23583194252956968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGCC952.635127E-514.2097473
GGTAGTC851.6327389E-414.1176747
CAGGACT902.652079E-413.3326034
GTATTAG2001.2732926E-1012.7521931
TACTGTG1551.13117494E-712.5806687
TTATACT3250.011.9993424
AGTGTGC1151.5775736E-411.7391538
GGGTAAT1303.8343835E-511.5404461
TTACGGT1059.7113336E-411.4279454
TAGGTAG1202.3441236E-411.2493845
TAGTCAG1100.001432086710.9091129
TAATACT4250.010.5876554
GCCTTAT1501.6391448E-410.001721
CCCTTAT1356.9407444E-410.0017191
GTCGCCG1356.951603E-410.00001930
CTAATAC2905.657057E-109.8270473
GTGTTAA2003.0302854E-69.75167751
TAGAACT2003.039073E-69.7494664
GTGTTAG1901.7502603E-59.4753131
AGTACTG1603.144762E-49.3744865
Produced by FastQC (version 0.11.2)