FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_82.35200000039ab0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_82.35200000039ab0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177957
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6440.3618851745084487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTT400.006489746718.7498255
GTATAGT550.00208846916.3680821
CTAATAC550.00209239716.3634833
GCAAAAA800.001646967313.12487813
GTACCAT1100.001419169110.9120551
GTATATA1100.001419169110.9120551
Produced by FastQC (version 0.11.2)