FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_81.35200000039a7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_81.35200000039a7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380101
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4490.11812649795712193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG402.4648313E-422.4979291
CGTACAC350.003001808721.4294173
GTCTTAG608.233967E-619.9981581
TATACGG908.5417923E-716.6673245
ATACGGT755.603107E-516.0006316
GGCCTCA600.00374678315.00059219
GTAACAC600.00374678315.0005923
GGTAGTC809.721579E-515.0005927
GTATTAA1002.5649824E-614.9986181
TTAGGTA952.6250173E-514.2110884
AATACTA1802.1827873E-1114.1672265
TAGGTAG750.001015442114.0005525
GCAGATC750.001015442114.0005528
TACGACA650.006374020613.8467014
TAGTACA800.001652367413.1255194
GTTCTAG800.001654005513.1237931
GTCTTAT800.001654005513.1237931
ATACTAC1151.0879339E-513.0439936
TAATACT1702.8021532E-812.3534294
CTAATAC1006.429352E-412.0004733
Produced by FastQC (version 0.11.2)