FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_80.35200000039a3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_80.35200000039a3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260819
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7180.27528669307067355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTA200.005140050329.9999984
CTAGTAC551.4103716E-724.5454543
TGAGTAG350.002999997721.4285725
TAGCCTG350.002999997721.4285725
TCTAGTA608.21116E-620.02
GTCTAAA559.5432406E-519.0909081
TGGAGGG400.006497321718.74999825
CTGTGTT400.006497321718.7499989
TAGTAGG400.006497321718.7499984
TCACACA500.001107097618.00000230
TTAGATA550.002095787616.3636364
CTGTGCA550.002095787616.3636369
GCTGCAG653.4238916E-416.15384728
GTATTTA859.148816E-615.8823531
GTACATA600.003743566615.01
CCTAATA600.003743566615.02
ACCCTAC600.003743566615.06
ATACTAT1503.0013325E-1015.06
GTATAGA1206.801383E-815.01
TATAAAC600.003743566615.02
Produced by FastQC (version 0.11.2)