FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_79.35200000039a03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_79.35200000039a03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721617
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19010.2634361441041439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTAG859.17887E-615.88506828
TTAACCT901.5804833E-514.9994474
GGTCGGG600.003753041214.99944614
CTAATAC2000.014.2494743
CTATACT1608.021743E-1014.061984
ACACCGC650.00638457613.8456428
TATGAGC650.00638457613.8456423
TAGGTAG902.6506322E-413.3328425
CGATCAC902.6506322E-413.33284224
CGCCCGT800.001655828313.12451612
ACCTAGC1056.732201E-512.85934229
TAGACAG1056.7453286E-512.85666755
CTATTAA1302.7554888E-612.6918391
TACAGCG850.0026113612.3524867
CCTAGCG1101.04019855E-412.27482530
CCTGTGG1101.0422203E-412.2722738
TCAGAAC1354.2033807E-612.221773
GTCTAGT1006.445845E-411.9995571
TATACTT3000.011.9995575
CTAGGCA1252.5714955E-511.99955710
Produced by FastQC (version 0.11.2)