FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_78.352000000399ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_78.352000000399ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380016
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44450.18676345032974567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1151.5805633E-411.7384896
CTAGGAC1355.656258E-511.1105053
GCGATTA1100.001433810910.90895413
TCTATAC6350.010.8655493
ACGCCCG1000.00878527510.49942611
GTGTCCC2105.2590076E-79.9994549
CTAATAC6050.09.9168143
TAATACT10850.09.8151324
CGTGTCC1250.00414487679.5994768
GTATTAG5500.09.5459361
TATTAGG5550.09.4591412
ATCGTGC1757.431546E-59.4280578
ACACAGT3552.7284841E-119.2952676
ACTGGGC2601.03431375E-79.2302658
TAGGTAG2957.941708E-99.1520425
ACAGTCG1654.2866246E-49.0904128
GGACAGC1500.00182510688.9995096
CTTACAC3001.0591975E-88.9995093
ACCGCAT2003.222136E-58.9995088
ATAATAC11350.08.9862933
Produced by FastQC (version 0.11.2)