FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_77.3520000003998b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_77.3520000003998b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656260
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28010.1691159600545808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC600.00375525414.99975911
CTAGGCA750.001018402513.99977510
GTATTAG4050.011.4819891
CGTGAGC1059.716051E-411.42838812
GTCTTAA2607.6397555E-1110.9620241
CTATGCA4000.010.8748259
CTAATAC4800.010.6248293
TAGGTAG2156.452319E-810.4649475
TAATACT9250.010.3782114
CCGTTCC1451.1673106E-410.3446619
CGGCATA1901.578348E-610.26330225
GTATTAC3550.010.14129451
CGATCAC1200.002954688210.00014124
TAGTCAG1952.202296E-69.9998399
TACCCGG1552.287196E-49.67755622
CCCCTAT1409.7101886E-49.6432841
GTCTATA3502.0008883E-119.4289881
TAGATAG3502.0008883E-119.4284195
GTATTAA6050.09.4219051
ACTATGC3851.8189894E-129.3504998
Produced by FastQC (version 0.11.2)