FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_76.35200000039948.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_76.35200000039948.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477037
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9540.19998448757643536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATG350.003002936321.4293961
GACTGAT350.003004763321.4271497
AACACCG400.006503598818.750725
GATAGTC400.006507536418.7487557
TAGACTG550.002098680216.3642655
TAGATTG653.430342E-416.1544675
CCAATAC1301.1204975E-815.0005773
GTATTAA1658.185452E-1114.5460141
CTAACCT851.6297524E-414.1181894
TACACTG650.006377206613.8466865
GTATAAT2350.012.7664491
TATACAG2255.456968E-1212.6671535
ATACCGT1354.196336E-612.2214116
ATACGGT1006.440382E-411.9992046
TAACACC900.00400006111.6671164
ACTGATC900.00400319611.6658928
GTTGCTT900.00400319611.66589211
AAACTTA1202.340085E-411.2492536
TAGAACT950.005986183411.0530564
ACAGAGT950.00599085711.0518978
Produced by FastQC (version 0.11.2)