FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_75.35200000039904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_75.35200000039904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3166646
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59130.18672753443232998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG5900.011.695631
TAGACAG4000.011.6244255
ACTGTGC2651.2514647E-910.1878538
GTCTATA6150.09.7566941
ACTGCGC1705.4141266E-59.7050958
ACTGGGC2803.243258E-99.6420768
CTTAGGC1250.0041453439.5995253
GGACAGA2054.1758103E-69.5117246
GCACCGT1757.433214E-59.4281046
ATGGCGC1801.01128026E-49.1659238
CTAGAAC4600.09.1299833
CCGAGCA1350.0078099568.8881689
ACCCTCC2201.02331E-58.8629188
TAGGCAG1705.776192E-48.8230935
CTGGGCA2403.2374282E-68.749299
GTATATA18700.08.5834111
TCCTATA5250.08.571142
TCTATAC8800.08.52230553
TAGAACT6350.08.5035164
ACTGCCC1952.4111684E-48.4608528
Produced by FastQC (version 0.11.2)