FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_74.352000000398cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_74.352000000398cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483330
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28740.19375324438931324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG750.00101930713.9979715
GTCCGCA954.1938166E-412.62974811
ATTAGGC1501.0410531E-611.9986663
AGGCCGT1151.5811237E-411.7374296
CTATGAC1951.5343176E-811.5371783
ACCCTGG1059.725495E-411.4269158
TAGACTG1459.350419E-611.3776615
GGCCGTC950.006004469511.051037
CAGTACT1602.7797907E-510.3110064
TAAGGCT2053.77986E-710.2424174
CTATACG1501.6461141E-49.998554
CGTAATA2454.1351086E-89.7948282
GTATTAG4950.09.6971991
CGCAAAG1250.00414525869.59893212
GGGTATA1757.4201234E-59.4287941
GTATAGA3851.8189894E-129.350871
TATACCG2105.6726294E-69.28436855
TCCTAAA2601.03376806E-79.2297432
AAATGCG1300.00572864539.2294325
GGACTAT1654.288075E-49.0895916
Produced by FastQC (version 0.11.2)