FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_73.35200000039899.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_73.35200000039899.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729183
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23670.3246098715960191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA802.4954534E-718.7486291
TCCTATA706.0364604E-414.9989022
CTAAGAC750.001017881213.9989773
CGTAATA650.00638619113.845142
GTTTATA902.6515036E-413.3323581
TCCTAGA800.001656308813.124042
TAATACA1302.7567185E-612.6913794
GGATATA954.1867403E-412.6306551
CCTTATA850.002612115112.3520372
TTATAGT1101.04260114E-412.271834
GTATAAA1101.04260114E-412.271831
TCTATAC1101.04260114E-412.271833
TAGTACT1006.4479513E-411.9991224
TATAAAT900.004006870611.6658132
TCTACAC1303.841867E-511.5376173
TATACTG1303.841867E-511.5376175
TAGACAG1059.7092934E-411.4277355
TTATACT1202.3434628E-411.2491784
AGCACAG1355.644872E-511.1102985
TGGACAG1355.644872E-511.1102985
Produced by FastQC (version 0.11.2)