FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_72.35200000039856.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_72.35200000039856.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length36
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTTGGTCGTTATTATGATGCTAGTATCATGGAGT11.3513513513513513No Hit
GGTATTTTGTTTCAGCACCTTCCAATTGTATGGCCA11.3513513513513513No Hit
CACAAAATTAGACTTTTTTTTGAACTTTGGAGCCTA11.3513513513513513No Hit
AATAACAGGGCGGAGTTTGCGCTCAAACAAGTAGGG11.3513513513513513No Hit
ATGATAAGCGATCTATATAAACGTTTCAATTAAGTT11.3513513513513513No Hit
ATGGGGTACGAGAACGGGATAAGATGGCATACAATC11.3513513513513513No Hit
AATGGTCAGAGAATCATAAAAGTATATAACAAATAG11.3513513513513513No Hit
TCCTGACATACATCGAATACTTGCGCAAGCCAAAGC11.3513513513513513No Hit
TTTGGTGGCTTTGCTGGTGCAGCATCCTTTTCACCC11.3513513513513513No Hit
GCAAAGTCCTGGAGGAATCTCAGATAGCCCACAGAC11.3513513513513513No Hit
ATATGAGTATACATAACAAATTTATAAAACTTTGCA11.3513513513513513No Hit
GGATTTTACCTCTATTAAAATCATTCAATATTTGCT11.3513513513513513No Hit
CTCTTATCTTTCCAGCTGACAAGACAAGTTTCTTAG11.3513513513513513No Hit
TAGGTGATGTGAATTGGTAGGGGGGAAAGACAGTCA11.3513513513513513No Hit
TTTCCATACACTTTCATCGACTCTCTCCCATACAAA11.3513513513513513No Hit
GTGGAGAATAAGGTGCATACGATGAAAAAGGTGATT11.3513513513513513No Hit
GAAGAATCATCTTCTTGCACGGGTACACCCAAACGT11.3513513513513513No Hit
GTACTGGTTTCGTCAAAATTAGTCGAGCTGGCGAGA11.3513513513513513No Hit
ATATAGATGAAGTAATCCTTGTTGGTGGTATGACCC11.3513513513513513No Hit
GGCAAAGGGCATATATAATTTCCCATTCGTCATGTA11.3513513513513513No Hit
ATACAGGTTGTTGCGTTTCTGTTGTGGAGATTTCAT11.3513513513513513No Hit
ATATAGCATTTCTAATGTCTCTAATCTGTTTGTTTG11.3513513513513513No Hit
ATTCTGGCGGATCCGTATCAATGGAGGGTGTATTAT11.3513513513513513No Hit
ATAAAAAACTATACAGAAGAAGAATTCCAAAACATA11.3513513513513513No Hit
GCTAAAGTCCTCATAAGTCAAAGAAATACCTGGCAG11.3513513513513513No Hit
ACTTGAACCTGGAAACTTCTGGATCGACGCCAGACG11.3513513513513513No Hit
GTGAAAGAGAGAATACCTTGATACCTACAATTGTTG11.3513513513513513No Hit
GGAAGACTCTAACACTACTGAAAACTCTGGTGCCAA11.3513513513513513No Hit
TATACTAGAAGTTCTCCTCCAGGATTTAGGAATCCA11.3513513513513513No Hit
CACCATTGAGAGCCACCGGTGAGTGCAATTCCTCTA11.3513513513513513No Hit
TTTCAAGACTGCCCACTTTAATTGTCAGACCTCGTG11.3513513513513513No Hit
GGATATCACTACAAACTGCGGCCTAAGAGGATGATA11.3513513513513513No Hit
GAATGAGCGTTCAATGTCATTCCGGCATGGGAAATG11.3513513513513513No Hit
GAATTAACAACAACAAATACGGTCACAAGAATGGAA11.3513513513513513No Hit
CCTTTAATGATGTCGAATTCGTGTGCAATAGAGCCA11.3513513513513513No Hit
GGGGGAGGTTGTCCAGGGGGAGGTGGGCCGTACGGG11.3513513513513513No Hit
TTACTGGAGTGTGGCCTGAAGTATTAGGAAACGAGG11.3513513513513513No Hit
CTTCCGATGATTCGAACTGTAATTGCAGACTACTTA11.3513513513513513No Hit
AGTCAAGTATGGAGACGAGAACGTAGCTTATGATGA11.3513513513513513No Hit
CCTAACTACTCTGCCACCACAAACTCATAAATCTCC11.3513513513513513No Hit
GATTAGTGGTCTAACTAATAGAAGATCCAAACCATA11.3513513513513513No Hit
ATATGGATCACTTCCAACAATTCCACTTGCTTCCAC11.3513513513513513No Hit
TTCCTATTATGTCGGAGGTGTCGGAGTATATTGGGG11.3513513513513513No Hit
GAATAAGCAAAAAGCAGGAAGAAACATCAATAATGT11.3513513513513513No Hit
GTATTAAACATGGTTATATGCTCCTAGTAGGTATTT11.3513513513513513No Hit
AAGTGAAAGTGCATCCAAACCAAAAGACGATGAATT11.3513513513513513No Hit
AAGCAACAAACTTCAACCTGTCGTCGAGGCTCAACT11.3513513513513513No Hit
ATTACAATTACAAAGTCGTAAAATTCAGGATTCTAA11.3513513513513513No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG11.3513513513513513No Hit
TAAGGATACTTTAACCGTCCCTACTTGTACTTCCAC11.3513513513513513No Hit
GTTATCAACATTTTATAATTTTACGCTCGGAAACAA11.3513513513513513No Hit
GCTTTACGAATATTATCTTCAGTATCAATTTTCAAG11.3513513513513513No Hit
GAAAAAAGAGCAAGTTAGAACGACAGAACGTTGCAG11.3513513513513513No Hit
TGCTACCAGCCATAATAACAGGAACAATGTTCTAGA11.3513513513513513No Hit
TTAAAAAGATGAAGCGTAGCGGTGGAATTAGAGGCA11.3513513513513513No Hit
CTCGAATGCTGTTCACATTCTCATTACGGTGGTGAA11.3513513513513513No Hit
AAAATAAAAAGTGATTTATCGTGGGCTTTATTTGTA11.3513513513513513No Hit
GCGTAAACGAAGAAGAACAGACCGTCGACAAGAACG11.3513513513513513No Hit
GAAAACACCGCGTTCTAGCATGGATTCTGACTTAGA11.3513513513513513No Hit
ATAATAATAATGCCAATACTATTTACTATAGAGCAG11.3513513513513513No Hit
GTACCATAGTGAACAGCAACAATGGAAGTACAGTTG11.3513513513513513No Hit
ACATAATAGAAAAATATATAAACTGGGCATCTTAAA11.3513513513513513No Hit
CACCATTATTTTTAGGTGCCCAATTGTATGTCCCTA11.3513513513513513No Hit
CCAAGAAGCGTTATGGGGAACGCTACGGACCACTGC11.3513513513513513No Hit
TGCAATAGCAGTTAACCTACCAATGATAATAACAAC11.3513513513513513No Hit
GTAAACAACAATAGGTATGGACTACGAAAGAACGGT11.3513513513513513No Hit
AAAGTGAATTTAACCGTTTGTTCAAAAGCTTTCTGG11.3513513513513513No Hit
ATATTTTAAATGGGTTGGGTTGGGGTGGTTATGATT11.3513513513513513No Hit
CTTTATTACCTTTTGAAAAGTGGAAATGTTGAGTTA11.3513513513513513No Hit
GATTTGTTGCGAAAAAAAAAAAAAAATTAAGAAAGG11.3513513513513513No Hit
TATGCAGGATGCCAATTGGGATCATTGCTTGTCTCT11.3513513513513513No Hit
ATGGATGATGGGGATAATGGCTAATGATCAATGTAG11.3513513513513513No Hit
TTCGTTAACTTAGAGAAGATAAATTGGATTTTAACT11.3513513513513513No Hit
GAAACGAGAAAATTATGACGCTTTAAAAATCGAAGG11.3513513513513513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAAGT50.029.99999810
AAATTCA50.029.99999821
AGGATTC50.029.99999827
AATTCAG50.029.99999822
TAAAATT50.029.99999819
TCAGGAT50.029.99999825
GTCGTAA50.029.99999815
TTACAAT50.029.9999982
TTACAAA50.029.9999988
GGATTCT50.029.99999828
AATTACA50.029.9999986
CAATTAC50.029.9999985
TACAATT50.029.9999983
TACAAAG50.029.9999989
AGTCGTA50.029.99999814
CAAAGTC50.029.99999811
GATTCTA50.029.99999829
AAAATTC50.029.99999820
CGTAAAA50.029.99999817
TCGTAAA50.029.99999816
Produced by FastQC (version 0.11.2)