FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_70.352000000397dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_70.352000000397dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586183
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12730.21716767630586353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT755.6209763E-515.9974524
GCATATG750.00101746913.9989651
TATATGT650.006384250713.8451312
CTCCTAT650.006384250713.8451311
TATATAC1251.7753555E-613.1978983
GTCTTAT800.001655642913.124031
TAGTACT800.00165670713.12291054
GTATAGA1056.7434645E-512.8561921
TAAAGTA954.187602E-412.6295685
CAGGAGC1006.449289E-411.9980894
CCTCTAT900.004005291511.6658041
CATATAC1303.8422426E-511.5366253
TATATAG1202.3419881E-411.2491672
ACACCGC1202.3438434E-411.2482086
TCTTATA950.005993962811.0518142
ATAAGGT950.005997770511.0508724
CTAATAC950.005997770511.0508723
ATACTGC1100.001432769710.9073546
TAGGAGA1000.00877882610.4983284
TACACTA1000.00877882610.4983285
Produced by FastQC (version 0.11.2)