FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_67.35200000039713.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_67.35200000039713.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861920
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15810.18342769630592168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC706.036282E-414.9994788
CTGTGCA706.036282E-414.9994789
GTATATA3250.014.7695751
CGTACAC1106.9112157E-613.63509753
TAGGGGT1004.2718057E-513.4987474
ACTCCGT800.00165625613.1245438
CGTATAC800.001656979713.1237823
CTATACA1551.1324482E-712.5794774
ACAGGTG1006.4479717E-411.9995828
CTGGGCT1006.4511166E-411.9988864
ACTGATC1901.0410076E-811.8416938
CCTAAGA1406.315284E-611.784622
TTAGGGC1151.5780321E-411.7387229
GACATCG900.00400673711.6662617
TCCTAGA900.004008474311.6655832
CCTATAG900.004008474311.6655833
CCCATGC1059.7093126E-411.4281748
TATACCG2103.7343852E-911.4275115
CAGATCA1355.6454326E-511.1107249
TAATACT3650.011.0948594
Produced by FastQC (version 0.11.2)