FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_65.352000000396a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_65.352000000396a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934979
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28890.14930394593429694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAGG908.584666E-716.6676271
CTAGTAC1406.325192E-611.7848713
TATACAG3700.010.9451645
TCTTATA3600.010.416192
CCTAAAC1304.920819E-410.3838733
CTAGGAG1756.6217253E-610.2849793
TAGACAG2505.2041287E-910.1992715
TCTGTAC2251.2562123E-79.9992853
TATAAGA5300.09.6222022
CTAGCCT1250.0041448639.5993144
CATTAGA3001.0531949E-99.4995662
GTATAAT3801.8189894E-129.4742311
TAATACC3358.185452E-119.4023124
GTATAAC2403.2067692E-79.3755421
CCTACCC1603.1496407E-49.37433053
GCGTGCT1450.00133966859.30991911
TAGGACT1450.00133998469.3096794
CCCCTGG1300.00572042269.2313021
CTTAACC1801.0106322E-49.1660113
GTACTAG1654.2808903E-49.09143451
Produced by FastQC (version 0.11.2)