FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_64.35200000039664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_64.35200000039664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233133
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22120.17938048856043914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTG550.002102064216.3632839
TACACAG1901.0426447E-811.8418495
GTCTAGT1652.554043E-711.8184041
GTCCTAA1406.3136104E-611.7859371
AGTGTCC1806.262417E-811.6664148
GTACTAG1703.760979E-711.4708051
CTATGCA2503.8198777E-1111.3997549
ACTGTCC2002.2255335E-811.2497578
TGTCCCT1755.4744487E-711.14261610
GTATTAG3100.011.1292421
CCGTCGC950.0059979911.0523929
CCAGGAC1100.001433069610.90885453
GGATAGA1951.8942592E-710.7694351
TATTAGG3250.010.6151552
TAGTCAG1704.677344E-610.5880079
TACGGTG2156.4463165E-810.46489057
GTCTAAG1756.608305E-610.2859091
GTGTCCA1756.611501E-610.2854929
CATACTG2209.026189E-810.2270525
GTTATAC2557.183189E-99.9997843
Produced by FastQC (version 0.11.2)