FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_63.35200000039621.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_63.35200000039621.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876890
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29340.15632242699359045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCC850.002612419412.35351221
GTCTAGT1859.159157E-811.3518741
GGCTCGG2002.226443E-811.250521
GTCTAGG1653.2723256E-610.90959551
CCCTAAG1150.002075004910.4352651
TGCGCCG1150.00207776510.43359610
GGGGTCG1304.917752E-410.384541514
GGTGAGC1602.7799098E-510.3113288
CTCGGAG2505.2059477E-910.1988413
TAGACTG1501.6461233E-49.9988635
ACCGTGT1653.900517E-59.9988638
CTTAGGG1501.6461233E-49.9988633
CTAGGAC1501.6461233E-49.9988633
CTGTGTG1952.205632E-69.9988639
GTATAGA4300.09.7678931
ACACAGT3254.5474735E-119.6912066
TAGGTAG2505.6546924E-89.5989095
GTATTAG4250.09.5298521
TTGCGCG1300.00572083159.23119519
TGGACCG1300.00572836079.229725
Produced by FastQC (version 0.11.2)