FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_62.352000000395ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_62.352000000395ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084571
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21570.19888047900967296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCT809.74151E-515.00177722
GTATTAG2400.015.0003921
GTCTAGA1206.8675945E-815.0003921
GCGCGCC650.00638073813.84779321
CTGAGTG800.00165770213.12352859
CGCCGCG1151.0914797E-513.04502224
TAATACT4650.012.9017784
GGCCAGC1354.2124266E-612.22085211
ACTGTGC1354.2124266E-612.2208528
CTGTGCC1006.454538E-411.9986539
TCTACAC2800.011.7843923
ACTCCGC1703.7657628E-711.4693018
GCCGCGT1202.3423061E-411.25081325
CACGCAG950.005997127411.0524116
CCCAGTT950.006001244311.0513922
CCTACAC1100.001433911810.90786653
GTCTTAC1253.420175E-410.80028251
GTACAGG1408.1700186E-510.7145651
GTGTACG1408.1700186E-510.7145651
CTAAGAC1408.181499E-510.7130833
Produced by FastQC (version 0.11.2)