FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_61.352000000395a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_61.352000000395a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514982
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27770.18330250788458213No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCG455.467054E-420.00033223
CCGCCGT650.00638658613.84638224
GTATTAG3850.013.2478471
GACCTCG800.001656624513.1252177
TAGGTAG1601.7133243E-712.1877025
CACCGCG900.004007595611.6668623
ACTACGC1059.715248E-411.4283838
ACAGACT2002.2269887E-811.2498148
CACCGTG950.00599736111.0528147
ACTCCCG950.005998834511.05244820
ACGGTGT1901.3250065E-711.0524488
CTCCTAT2053.1997843E-810.9765161
TAGACTG1807.8745325E-710.8335125
CTAGGAC1408.175748E-510.7144623
TATACCG2301.5488695E-810.4349555
CCCTATA3051.2732926E-1110.328042
CCTATAC3350.010.29867653
TATTATA12200.010.0821342
CTACTAG2105.2436917E-710.0008251
GTCTAGA1952.1990545E-610.0008251
Produced by FastQC (version 0.11.2)