FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_60.35200000039565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_60.35200000039565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685676
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14130.2060740057986571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAG550.002099593416.3646722
GTCAGAT755.61602E-515.999845511
TAGACTA1054.264133E-614.2855755
GACCGTC800.001655341913.1248737
AGACCGT954.183935E-412.6314556
ACCGTCG954.1865098E-412.6305358
CTATACT1501.0370713E-611.9998854
GGTAGTC1006.443649E-411.9998837
AGTCAGA1006.4476003E-411.99900810
GTATTAT3550.011.833461
TAGTCAG900.00400673911.6657029
GTCTTAG1059.68508E-411.4309621
GTACAGA950.005983107311.0549421
TATGTAC1501.35716E-510.9998943
GTTCTAA1653.2560765E-610.9113731
AGACTAC1253.4178438E-410.7998956
TCTTATA1551.9493638E-510.6458352
GTCTAAT1704.654654E-610.590451
GTATAGA1704.654654E-610.590451
AACGATC1000.0087669110.50066427
Produced by FastQC (version 0.11.2)