FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_59.35200000039522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_59.35200000039522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172944
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16940.14442292215144117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG550.002103654216.3613367
GCCCGTC600.003756432314.99852911
GTATTAG2009.094947E-1213.4986771
CAGTGCG800.001658198313.1231559
GTTTAGG850.00261423912.3517291
TAGGCTA850.00261507512.3512035
GGTAGTC1006.456771E-411.9983127
ATCGTGC900.00401138111.6650268
TAGACTG1551.5457063E-611.6112695
CCAGTGT1202.3472488E-411.2484178
AGGACAG1653.2762418E-610.9075575
CGCCCGC1000.00878364110.49897112
TCCACCG1304.920859E-410.3831555
GGCGAAC1501.6432532E-49.99987215
GTGTAGT1952.2025542E-69.999021
CTAAGAC1653.8982835E-59.9985933
CAGTGTG1356.96436E-49.9985939
GGACAGT1952.2037566E-69.9985936
CTAGCAC1356.96436E-49.9985933
TAGAACT2905.693437E-109.8262034
Produced by FastQC (version 0.11.2)