FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_58.352000000394ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_58.352000000394ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744385
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15520.20849426036258117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC1054.2697375E-614.2841463
TTAGTAC750.001018198113.9984633
TAGTGTG750.001018198113.9984637
GCGCCAA650.006387903413.84463511
ACTGTCC1004.2709496E-513.4985198
TATACGG1701.9899744E-913.2338415
AGTGTGT1151.0929511E-513.0420478
TAGACAG1056.750485E-512.8557325
TAGGTAG1056.750485E-512.8557325
ACCATGC1651.8617357E-812.7258768
TCTTATA1652.5530608E-711.8168842
ATACGGT1551.5426267E-611.6116296
TACGGTG1059.712684E-411.4273187
TTAGAAC1459.329327E-611.3780623
GTATTAA2259.094947E-1011.3328511
CCTAATA1602.260198E-611.2487652
TACACAG1202.3443835E-411.2487655
ACGGTGT1202.3443835E-411.2487658
CCCTTAT1355.643481E-511.1106391
TAGTACC1901.3227327E-711.0514194
Produced by FastQC (version 0.11.2)