FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_57.352000000394aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_57.352000000394aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531015
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC400.006506934818.74952911
GTGTAAG703.105485E-517.1440411
TTAGGTG550.002099992716.3632265
CAGACCC653.4329205E-416.1534399
ATACGGC1002.567851E-614.9996246
TCACACA1104.188605E-714.99962330
ATCACAC1104.188605E-714.99962329
TCCTAAG600.003751006714.9996232
CGCAGAT650.00637716913.8471118
TAGACTG650.006381148513.8458055
TACAGAC1201.1193151E-613.7496557
CTATGCA1752.0918378E-1013.7139429
AACGCTT1004.2630447E-513.49966124
CAGATCA800.001654678413.1246719
ACTATGC1507.353265E-812.9996738
CCTTATA954.1783965E-412.6324521
GTATTAG1201.6880056E-512.5008631
CATCACA1354.1969233E-612.22191628
CTTATCC1252.568182E-511.9996993
TACTATG1754.213689E-811.9996997
Produced by FastQC (version 0.11.2)