FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_56.35200000039466.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_56.35200000039466.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795089
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16490.20739816548839188No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGCC601.8661999E-417.4982384
TAGGTAG1553.092282E-1115.4823145
GGTTAGG600.003753507714.9994341
ACTATGC2250.014.6651918
TGGACTG1351.8522769E-814.4429915
TAGTCAG1156.943792E-714.3463829
TATTAGG2150.013.9520842
GCCCGTT650.00638270113.84650226
CTAATAC1651.2732926E-913.6349923
GGATAGC902.651182E-413.3328291
GTCAGAT1701.9899744E-913.23396211
GTATTAG2500.013.1995021
ACTGCGT800.001656880413.1236798
TAGACGG800.001656880413.1236795
GCTCGTT800.001656880413.12367911
GTACTAC1302.756513E-612.691831
AACACAG1302.7584574E-612.69103055
GGTAGTC1456.8950794E-712.4125447
CTAGAAG1652.5535883E-711.8169933
GTGTTAG1151.57778E-411.73868751
Produced by FastQC (version 0.11.2)