FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_55.35200000039423.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_55.35200000039423.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083507
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19180.17701777653490008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC500.001110419717.9993926
CTAGGAC600.00375342815.0001853
TAACGTG809.7541444E-514.9994935
GCTGACC600.003754580614.9994938
CGGGTCC750.001017429514.00081825
GAGCGCA750.001018139713.9995269
GGCCTCA1507.381823E-812.9995619
AGGCCTC1651.8633727E-812.72684218
ACTATGC3300.012.2723128
TACACCG1101.04312174E-412.2723115
TGAGGCC1857.017661E-912.16175116
CTAATAC3750.012.0001483
AGACTGT1551.5433598E-611.612516
GTATAGA2850.011.5806931
TCTAGGG1303.842533E-511.5386043
CTGGTCA1859.1535185E-811.3509679
GGTCCCA1202.3415979E-411.25117827
TGGACCA1355.64848E-511.1107355
GCTGGGA1100.001431317910.91023228
GTATAAC2402.6411726E-910.6266021
Produced by FastQC (version 0.11.2)