FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_54.352000000393ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_54.352000000393ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392013
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23550.16917945450222088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG1156.9567795E-714.3459885
CGCACCG750.001019121613.9982069
CGCCGCG1201.6935894E-512.50019524
CACCTAG1006.4457353E-412.00104928
GTCCTAA1406.3154803E-611.7858981
CCGTCGC1406.325603E-611.78420459
GCCGCGT1151.577573E-411.74015625
CGTAAGG1303.8484694E-511.5373983
GTATAAA5100.011.4707671
CCTACCC1059.720542E-411.4275183
AGTGTGT2103.747118E-911.4271078
TAATACT6450.011.1617614
ACCGTCG2155.4169504E-911.1613618
GGCTCGG1355.648317E-511.1112831
TAGACTG1355.656587E-511.1096885
AGTCCGG1501.3617335E-510.9985910
GGACAGG1100.001434544810.9076946
ACTCCGC1951.8988794E-710.7678518
TACTATA4900.010.7132982
GTCCTAG1000.00877762710.5001641
Produced by FastQC (version 0.11.2)