FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_53.352000000393ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_53.352000000393ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514121
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9160.17816817441808447No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCG8550.16630326323958758No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACG7530.1464635756952157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTGC200.005146763829.997478
CCTAGCG455.45686E-420.00220530
TCCTAAG651.642842E-518.4617792
CACGCAC500.001108991918.00023527
CGATCAC500.00110971617.99848424
CCTTAGT550.002099335416.363852
CTAAGGG550.002100700316.3622594
TAGGCAG653.4316565E-416.15405711
TAGGTAG859.190835E-615.8810155
GTGCGAC1801.8189894E-1215.00165429
GGTGCGA1801.8189894E-1215.00019528
CTAGGCA600.003752265814.99873510
TGCGACG1955.456968E-1213.84768130
AGTCAGA1201.1198608E-613.74884110
GTATTAG1557.72161E-913.5485631
TCTTAAG902.6471916E-413.3335072
TACAGGC2500.013.1988877
CAACACG2300.013.04491713
GTTCTAT1404.4893932E-712.8573091
AGGTGCG2102.1827873E-1112.85730927
Produced by FastQC (version 0.11.2)