FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_52.35200000039374.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_52.35200000039374.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975108
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17940.18397962071893575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACTG653.4391688E-416.151865
AGGGTCG706.041334E-414.9981558
GTGCGAC1054.262258E-614.2876229
CAGACCC1004.274147E-513.4983399
TATACGG1905.4569682E-1113.4194015
GACCGTC902.6540615E-413.3316947
TCCTAGA1251.7780876E-613.1983762
TGCGACG1151.09104185E-513.045217530
CTAGACA1151.0939282E-513.0418744
GTATTAG2550.012.9402481
TCTAGAC1302.7609713E-612.6907463
GTCCTAG954.188944E-412.6306721
CTAGGAC954.1907563E-412.6300243
TCTACAC1804.6748028E-912.4984633
CACGCAG1501.0380554E-612.00036916
GTACTAA1501.0393433E-611.9991391
TAGGCTT1252.5759417E-511.9985245
ACTAGAC1406.3198004E-611.7842643
CTAATAC3200.011.7173083
TTAGGCT900.004010023511.6652324
Produced by FastQC (version 0.11.2)