FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_51.35200000039331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_51.35200000039331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621729
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29990.1849260881441967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCC850.002612376412.35337323
GACCGTA850.002614189612.3522297
CTCTTAG2002.2237145E-811.2510871
TCCTAAG1755.476686E-711.1429032
CCAGCCT950.006000973711.0519968
ACTATGC4350.011.0338498
GTATAGA5250.010.85819151
ACTTGCC1951.897879E-710.7686118
CTACGAC1000.00877942510.50004315
TTAGGGT2156.452865E-810.4648364
GATTAGG1304.912278E-410.3856191
GTAGGAC1451.1674017E-410.3445513
CTAGCCT1451.1674017E-410.3445514
GTATTAG4150.010.121461
GTCTAGG1809.239595E-610.0009661
GGTCTAA1501.6423213E-410.0009661
TCTAGGA2403.000605E-810.0000412
TAGGACC1653.8959042E-59.9997324
ACTGTTC4150.09.7584748
CTATGCA3850.09.7396999
Produced by FastQC (version 0.11.2)