FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_50.352000000392fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_50.352000000392fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940230
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17830.1896344511449326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAAGC800.001656503213.1244893
TAGACAC1056.753052E-512.8559585
CTCTTAG1151.5776053E-411.7392971
GTATTAG2206.2573235E-1011.5910741
TAGACTG1059.715416E-411.4275185
AGACTGT1459.334788E-611.3782626
GACTGTT1202.3452504E-411.2489637
GTATAGG1755.466627E-711.1430151
GTGAGCT950.005996970511.052213
GGTAGTC1501.3598899E-510.9989857
CTTATAG1653.2699954E-610.9086653
TAGGTAG1951.8950595E-710.7682385
TTAGGTA2402.6484486E-910.6240214
TAAGACA1704.677451E-610.587264
TCTTAGG1000.0087776210.4995912
GTATATG3300.010.4546941
CTAATAC2651.2441888E-910.1882813
ACCTAAT2802.9831426E-1010.1787161
TATTAGG3152.3646862E-119.999612
CATGTAC1356.955122E-49.999613
Produced by FastQC (version 0.11.2)