FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_49.352000000392ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_49.352000000392ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919126
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30960.16132343577232555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2151.8189894E-1213.25563055
GTATTAG4200.013.2158231
TAGGCTG1059.717105E-411.4284125
AGCAGTC2402.6557245E-910.6248527
CGTGAGC1851.1214488E-610.54039412
CGGACCC1000.00877988310.5001294
TCTATAC4150.010.4820553
TCGGTCG1150.00207607910.4346389
TAGTCAG2752.1645974E-1010.3634939
GTATAGA4350.010.3460321
GTCCTAG1602.7729582E-510.3137011
ACGATCC1602.7769434E-510.3123578
AGTGTGC1756.617087E-610.2855728
CCAGGAT2352.1655069E-810.2128913
CTCCTAA2251.252738E-710.00116351
TAGACCG3152.3646862E-119.9998615
TGGACAG3501.8189894E-129.8570065
TCTAAGC3101.8553692E-109.6775373
CTTAGAC2505.6439603E-89.6001173
CTAAGCA2854.3855835E-99.4737994
Produced by FastQC (version 0.11.2)