FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_48.3520000003925c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_48.3520000003925c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287886
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8980.31192902746225937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT350.003000745521.42857212
AAGTGTG455.4529007E-420.07
TAGGTAG500.001107477518.05
TAATACA601.8595539E-417.5000024
AGTAGAC601.8595539E-417.5000023
CTATACT805.1460174E-616.8754
ATACTGA1102.3366738E-816.3636366
TCTACTC550.002096498916.3636363
GACCCGA600.003744823215.00000122
TATACTG1156.891569E-714.3478265
GTATTAG851.6267409E-414.1176471
GAGTGAT750.001014693514.011
ATTAAGC650.006370730713.8461533
GTGTGGA650.006370730713.8461539
TAAGAGC650.006370730713.8461534
GTCTAAA800.001651163213.1249991
TACTATG1151.0861944E-513.0434797
GTATTAA1056.715657E-512.8571431
TTATACT1302.7380538E-612.6923074
TTATCCT850.002604064312.35294154
Produced by FastQC (version 0.11.2)