FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_47.35200000039218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_47.35200000039218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575517
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11150.19373884698453736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTGC350.003006724921.4256538
GTATAGG1156.9369344E-714.3458721
TAGGTAG851.633096E-414.11572555
CGGTGTT750.001017908213.9980959
GCTGCTC650.006386697813.844278
CTTTCGC902.6494474E-413.33267622
GACATAG902.651397E-413.33151830
TAGTCAG902.651397E-413.3315189
CCCATCC800.001654188813.12549513
ATAGGTA800.001656355213.1232153
TTAGGTA1456.887003E-712.4121034
ATTAGGT1501.0376934E-611.9983663
GTATTAG2154.2564352E-1011.85885051
CTAGACC900.00400700311.6650784
TAAGAGT1303.8410544E-511.5368914
ACGGTGT1202.3431987E-411.2484698
AAGACAC1755.463153E-711.141345
GTAGTCA1355.6437028E-511.1095998
GAGTAAG950.005996514611.0511271
GTAGCAC950.005996514611.0511273
Produced by FastQC (version 0.11.2)