FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_46.352000000391d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_46.352000000391d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392763
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8840.22507211728192317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAC400.006504160418.7494415
AACTATC601.8621577E-417.499487
CTGTACT650.00637744113.8457424
GTATATG1151.0889296E-513.0430911
ATCCCTA954.1730874E-412.63281218
CTCTATA954.1775728E-412.6312042
TAGAACC850.002607565112.3525734
CTGTGCT850.002607565112.3525739
GTTCTAC900.00399994711.6663211
GTCTATA900.00399994711.6663211
ACTATGC1059.6883404E-411.4282328
ATGATGC950.0059860311.0523038
TGAACTG1100.00142939910.9087675
CAATACC1253.4114576E-410.799684
ATATTAG1253.4114576E-410.799681
TAATACT2104.5251E-810.7139674
GGATAAT1000.00876175510.4996881
AATACCG1304.90024E-410.3843075
TCAATAC1501.6372236E-49.9997033
CTAAAGT1200.00294720739.9997024
Produced by FastQC (version 0.11.2)