FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_45.35200000039190.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_45.35200000039190.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268011
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG402.4606666E-422.4999989
ACTGTGC500.001107206618.08
GGTAGTC653.4243337E-416.1538477
GTCTTAT706.015477E-415.01
TCTATAC809.707832E-514.9999993
GTCATAT851.6260802E-414.1176471
TCTAGAG750.001014372514.03
ACCTAAT650.006369218713.8461531
TATCCAG650.006369218713.8461535
GTATTAG1352.868619E-713.3333341
GTATATG1456.829978E-712.4137931
TCAATAC1252.5563964E-512.03
CCTAATA1151.5697627E-411.7391312
ATTCAGT1151.5697627E-411.7391316
TTCAGGT900.003993393411.6666672
TCTCTAA950.005976303511.0526311
CTATACC950.005976303511.0526314
CTATACT1100.001426476510.9090924
CTTCTAT1100.001426476510.9090921
TATACTT1100.001426476510.9090925
Produced by FastQC (version 0.11.2)