FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_44.3520000003915d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_44.3520000003915d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425983
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6860.16103929030031716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT400.006507010718.748464
CTATACT859.183252E-615.8810464
GTATAAT1655.456968E-1215.4532741
CTTTAGG600.003750807614.9987672
TTTATAG750.001016730413.998853
CAGATCC650.00638082513.84501659
TACTGCA650.00638082513.84501657
GTGAACG650.00638082513.84501659
GGAGAGT800.001654452613.1239226
CTATCCT1507.345261E-812.9989324
TAGAGCT850.00260921612.3519254
GCTATCC850.00260921612.3519253
GTATGCT850.00260921612.3519254
CATATAG1006.4387417E-411.9990143
ACGTAAT1151.5751386E-411.7381661
GGTCAAG900.00400246611.6657081
ATAACAC900.00400246611.6657083
GGTTAAT900.00400246611.6657081
ATACTAC1303.8334783E-511.5375141
TATACTG1303.8334783E-511.5375145
Produced by FastQC (version 0.11.2)