FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_43.35200000039133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_43.35200000039133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329985
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6270.1900086367562162No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG350.00300166521.4285745
GGGTATA455.4552255E-420.01
CGCATGG400.006500890518.75000230
CTCCGGT500.001107944218.00000229
AGAGTCC703.1015643E-517.1428598
CAGCGTG550.002097373616.3636369
GTCTTAG550.002097373616.3636361
AGGCCAG653.4273378E-416.15384710
AGCACCT755.601407E-516.015
TAGACCG755.601407E-516.05
GCAACTT600.003746372215.013
AGAGGCC851.6278803E-414.1176478
AACCCTT650.006373336513.8461536
GCTGGCT650.006373336513.84615324
TAAGGTT650.006373336513.8461534
TATTGTA902.6429837E-413.3333332
ATATTAT1601.1994416E-813.1250011
TATTATA1302.7415044E-612.6923072
TTAATAG850.00260545812.35294153
GTATAAA1101.0385559E-412.2727271
Produced by FastQC (version 0.11.2)