FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_42.352000000390fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_42.352000000390fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896065
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18420.20556544447110423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG1251.7736511E-613.2010071
GTATTAG1601.2085366E-813.1260011
GTCTTAG1354.20126E-612.2231551
CTATGCA1601.7126877E-712.1863899
GTCTTAT1059.7009377E-411.4294431
GTCTAGG1202.341354E-411.2508581
GTATAAA3650.011.0967371
ATACTCG950.005998927611.0516246
ATTATAC4250.010.9401793
TCTAGGA1253.4208663E-410.7996182
GTTATAC1408.1761034E-510.7133083
TACCGTG1408.1761034E-510.7133087
GCCTTAG1150.002072665610.4355781
GGTATAA2608.931238E-1010.3854071
GTACCGT1304.916184E-410.3836696
TATACCG1756.610466E-610.2847785
GTTCTAT2352.1560481E-810.2135441
CCCTTAC1356.947079E-410.0007631
TATTAGG2105.2418545E-79.9996472
CAGTGCA1356.9577765E-49.9990899
Produced by FastQC (version 0.11.2)