FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_41.352000000390ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_41.352000000390ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739997
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9210.12445996402688118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA200.00514654129.99945811
ACAACGC601.8638375E-417.5008668
TAGACTG805.165648E-616.8758375
CCGTCCG600.00375268814.99972929
TGGACAG1251.1301563E-714.4007145
ACAGTGC851.6315164E-414.1183468
TAGACGG750.00101696314.0006935
TCCGACT750.001017482713.99974812
TGGACTG1352.8900467E-713.3339945
AGTGTCG800.001655661613.124763510
TATACTG2102.1827873E-1112.857785
GTATAGA1804.654794E-912.5006191
CAACGTA850.00261109712.3527199
ACTCGAC850.00261109712.35271924
CTAACAC1101.0414472E-412.2733353
TAGAACT1601.7079947E-712.1881044
CCTTAGC1151.5762163E-411.7397121
GTTCTAA2301.05501385E-1011.7397121
CTAGCAC900.00400329611.6672453
CAGTGCC900.00400531811.6664569
Produced by FastQC (version 0.11.2)