FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_40.35200000039077.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_40.35200000039077.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336961
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6660.197648986084443No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCG3690.10950822201975896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG200.005143897629.9979231
TGAACCG400.0065037718.7487015
TAACTCA400.0065037718.7487016
CAGCTAA805.1546685E-616.8738339
TACGGTT550.002098468616.3625035
GTACTAG653.4294178E-416.1527271
TTGAACC653.4294178E-416.1527274
ACAGCTA859.170732E-615.8812528
CGCTCCT706.0175575E-415.00118829
TTCGCTC706.0175575E-415.00118827
CCTTTCG600.003744618315.00118624
TGTGTTC600.003748318414.99896053
CTGTGCT952.625964E-514.2095429
CTTTCGC851.6269476E-414.11876425
TCTGTGC851.6290006E-414.1166698
CTGAAGG750.00101471714.00110825
TGTGCTA750.001015856413.9990310
ATCAGGC650.006376633413.8451948
GTTATAA650.006376633413.8451941
TTTCGCT902.6414727E-413.33438926
Produced by FastQC (version 0.11.2)