FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_39.35200000039034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_39.35200000039034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617878
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11650.18854854841894353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGC400.00650787618.7496477
ACCATGC706.032126E-414.9997178
TAGGACT600.003751776414.9997174
ACTGGGC750.001017155113.9997378
TAGACTG902.649261E-413.3330825
GTCAGAT1352.8898467E-713.33308211
AGCGCTA800.001655133613.12475310
TAGGTAG1507.360359E-812.9997555
GTATAGA2600.012.6930961
GTATATA6750.012.4452161
GAGAGCC850.002610273412.3527089
TCTAACG850.002610273412.3527082
GTGAGTA850.002610273412.3527089
CATCCGA850.002610273412.35270810
TGTACAC850.002610273412.3527083
ACTTTGC1101.0415761E-412.2724958
GTATTAG2257.094059E-1112.0007451
ATTAGGT2001.7280399E-911.9997733
ATACCGA900.004004063611.6664476
GTAGTCA1303.837525E-511.5382448
Produced by FastQC (version 0.11.2)