FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_38.3520000003900d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_38.3520000003900d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681041
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11560.16974014780314256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCC550.002101525416.3623944
TAGTGAG750.001017780713.9989395
CTGTGTA750.001017780713.9989399
GACTAGG650.006382628313.8461211
CCTATAC650.00638573413.8451033
TATGTGT1201.1212906E-613.7489574
GTATATA2500.012.599971
CTCCATG1006.443156E-411.999971
CGAGGAA1006.443156E-411.9999714
TATACAG1252.5720205E-511.9990895
CTGTACT900.00400648911.6657814
TAGGGTT900.00400648911.6657815
GTATTAC950.005992464711.0526051
ACAACGG950.005995740611.0517928
CATACTC950.005995740611.0517923
TAAGCCT1100.001432289610.9082634
GTATTAG1253.4175493E-410.7999741
TAAAGGT1551.95231E-510.6443544
CATAAGA1551.95231E-510.6443542
GTCTAAT1000.00877107610.4999741
Produced by FastQC (version 0.11.2)