FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_37.35200000038fbe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_37.35200000038fbe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975667
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17980.1842841871253204No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG706.0391455E-414.9988725
CCTAGAC851.6340887E-414.1165863
TATACCG900.00400859111.665795
TATACTG2503.8198777E-1111.3991435
GTCTTAG1202.343937E-411.2497321
TCTACAC2453.1832315E-1011.019583
GAGGGCA1100.001433244310.9082729
GTCTAAC1551.953576E-510.6449061
GGGCAAT1000.00877703710.49974911
GCAAGAC1150.002075562310.4339983
GTATTAG3152.3646862E-119.9997611
GTATATA5550.09.9997611
GACACAG1200.00295501649.99924855
ACTATCC1653.893651E-59.9992488
GAGTTAA2301.7252569E-79.7823751
CTAGACA1250.0041418729.5992784
TTACACT2352.3609027E-79.5737484
TCTATAC2752.4650944E-89.272033
TAGGGTA1801.0093007E-49.16597755
CCTAATA1654.2817398E-49.0902252
Produced by FastQC (version 0.11.2)