FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_36.35200000038f88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_36.35200000038f88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504451
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15020.2977494345337803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG500.00110956617.9987533
GGACTGA550.002100417916.3625016
CCAGCCT550.002100417916.3625014
GGAGCTC600.003751764314.998968
ACTGCGC600.003751764314.998968
CTATCCT706.0317153E-414.998964
TTAGGAC650.006382434613.8451933
GTGGCTA650.006382434613.8451939
AGGACTG800.001655030213.124095
GTATTAA1507.326162E-813.0029651
TTAGAGT1201.6900116E-512.4991334
TATACTG1355.6366705E-511.110345
GTCTTAT950.005978548411.0551531
GTATTTA1501.3516226E-511.0025081
CCCTTGA1100.001427547210.9115791
CCTTATA1100.001431095910.9083352
TTAGACA1100.001431095910.9083354
ATTATAG1253.416348E-410.7992513
TATAAGA1551.9494082E-510.6444232
TTAACCT1000.00877013310.4992724
Produced by FastQC (version 0.11.2)