FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_35.35200000038f45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_35.35200000038f45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908132
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32040.1679129116853551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3350.012.5371821
AGACCGT1501.36187755E-510.9990216
ACCGTGC1000.00878644910.49906358
TAGACCG1150.002077367210.4338535
TTCTATA5850.010.2554972
GGACTGA1501.6457467E-49.9991096
GTAGGAC1200.00295756769.9991093
GTAGCGC1200.00295756769.9991098
GTATTAT6650.09.9247071
GTATATA9700.09.8968041
GTATTAC4550.09.8900061
CCTTATA4300.09.7665712
TATTATA8450.09.7624442
ACTAGAC2209.930754E-79.5446043
TAGACAG2854.3928594E-99.472845
ATTATAC5600.09.3741653
GTCTTAT3901.8189894E-129.2306721
CAGCGGC1300.00572727539.2299479
GACCGTG1300.00572727539.2299477
TAGACTG2651.3857243E-79.0557975
Produced by FastQC (version 0.11.2)