FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_34.35200000038ef3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_34.35200000038ef3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810360
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15600.19250703391085444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCAGG550.002099711416.365051
GTCTTAA1504.8821676E-914.0012091
GTTAGAC902.6512647E-413.332843
GTATTAG2301.05501385E-1011.7401451
TTATACA2607.6397555E-1110.9611334
CCAGCAC1100.001432334410.9086883
TCTAAAC1000.0087757410.4996113
CTTAAGC1150.00207425210.4343973
TAGACAG1304.912645E-410.3842315
TATACTG2752.1464075E-1010.3632535
GTTTTAG1200.00295014310.0008641
GTATATG3451.8189894E-1210.0008641
GTATAGG1356.9449097E-410.0008641
GTATTAT2905.638867E-109.8284361
GTATTAC1851.2769846E-59.7305711
GATTAAT1851.2769846E-59.7305711
GTATAAC1705.390794E-59.706721
TCTAGAT1552.2851198E-49.6770613
TAAACAG1250.0041392889.5996455
TATTAAG2352.357192E-79.5741132
Produced by FastQC (version 0.11.2)