FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_33.35200000038ebf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_33.35200000038ebf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297705
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3090.10379402428578627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGTA550.002097588716.3626272
GTAGTGT550.002097588716.3626274
GTATAAA851.6278878E-414.1167771
TCTAAAG750.001015309513.9991372
CTTCTAG650.00637400613.8453021
CCTCTAG650.00637400613.8453021
GAACTAT800.001652160213.1241911
TTGTATT954.1734194E-412.63084
GTGTATA954.1734194E-412.63081
GTAATAT850.002605629412.352181
GTACTGG850.002605629412.352181
ATAGTAC850.002605629412.352183
TTAGATT900.00399701111.6659494
TATTAAA900.00399701111.6659492
TACTAAT900.00399701111.6659497
CAATACT900.00399701111.6659494
GTATTAA1059.678886E-411.4278681
AACTCAT1000.00875546110.4993527
TAATACT1150.00206805310.4341394
TCATTAT1200.00294441039.9993841
Produced by FastQC (version 0.11.2)